474 



D. W. BRUNER AND P. R. EDWARDS 



components from a normally monophasic-specific species has 

 not been reported previously. For that reason the character- 

 istics of the non-specific components (phase 2) of S. paratyphi A 

 are given in more detail than are the characteristics of the "arti- 

 ficial" phases 3 and 4. The agglutinative relationships between 

 the non-specific components of S. paratyphi A and the non-specific 

 phases of diphasic types are given in table 2. 



It is apparent that the non-specific phases of S. paratyphi A 

 are more closely related to the 1,5... phases than to the 1,2..., 

 1, 6. . . or 1, 7. . . phases. The organisms were tested with ab- 



TABLE 2 



Agglutination of non-specific phases of S. paratyphi A by non-specific serums of 



other types 



Paratyphi B (1, 2. . 

 Newport (1, 2. ..).. 

 Kunzendorf (1, 5 . 

 Paratyphi A 228. . . 

 Paratyphi A WB39. 

 Sendai (1, 5...).... 

 Anatum (1, 6. . .). . 

 Nyborg (1,7...)... 



Para- 

 typhi B 

 (1,2.) 



10,000 

 5,000 

 2,000 

 2,000 

 2,000 

 2,000 

 2,000 

 5,000 



Newport 

 (1,2...) 



10,000 

 40,000 

 10,000 

 2,000 

 2,000 

 5,000 

 2,000 

 5,000 



Kunzen- 

 dorf 

 (1,5...) 



5,000 

 5,000 

 20,000 

 10,000 

 10,000 

 10,000 

 5,000 

 2,000 



Para- 

 typhi 

 A 228 

 Phase 2 



1,000 

 1,000 

 40,000 

 40,000 

 40,000 

 40,000 

 10,000 

 200 



Para- 

 typhi 

 AWB39 

 Phase 2 



500 



1,000 

 40,000 

 20,000 

 40,000 

 20,000 



5,000 

 200 



Anatum 

 (1.6...) 



2,000 

 2,000 

 5,000 

 5,000 

 5,000 

 5,000 

 10,000 

 2,000 



Nyborg 

 (1,7...) 



2,000 

 2,000 

 2,000 

 1,000 

 1,000 

 1,000 

 1,000 

 10,000 



Figures indicate highest dilution at which agglutination occurred. 



sorbed serums containing agglutinins for non-specific factors 2, 

 3, 5, 6 and 7, respectively. The preparation of these serums 

 was described by Bruner and Edwards (1941). The non-specific 

 components of S. paratyphi A were flocculated only by serums 

 containing agglutinins for factors 3 and 5. In this respect they 

 resembled the Kunzendorf, Berlin and Sendai types. Phase 2 

 of S. paratyphi A, therefore, may be expressed as 1, 5. . .. 



As mentioned above, the non-specific phases derived from cul- 

 tures of S. paratyphi A were not identical. The differences in 

 the phases were discernible only in agglutinin absorption tests. 

 It was possible to divide the phases into two groups whose non- 



iu 



