XIV CONTENTS 



Page 

 B. The Formation of Specific Inhibitors in Enzyme Reactions 160 



1. Pepsin Inhibitor 161 



2. Tr)'psin Inhibitor of Pancreatic Extracts 162 



3. Tr)'psin Inhibitor of Blood Serum 163 



4. Inhibition of Carbohydrases by the Reaction Products . 163 

 5-13. Similarly Formed Inhibitors 165-173 



PART IV 

 ANTI-ENZYME IMMUNITY 



A. Analysis of Certain "Controversial" Aspects of Anti-Enzyme Im- 

 munity 175 



1. Analysis of Bayliss' Objections Against the Existence of Anti- 

 Enz^Tnes 176 



a. The Nature of the Trypsin Inhibitor Present in Sera in 

 Relation to Anti-Enzyme Antibody 178 



b. The Nature of the Tr)'psin Inhibitor in Egg White 179 



c. The Resistance of "Living" Protein to the Action of Pro- 

 teolytic Enz)Tnes 180 



d. The Inhibition of Enzymes by Enzymes and Viruses 183 



2. The Question of Non-Specific Adsorption of Toxins or En- 

 zjones on Coexisting Protein-Anti-Protein Precipitates 185 



a. Adsorption of Toxins on Red Blood Cells and Charcoal 

 without Loss of Activity 188 



b. Failure of Protein-Anti-Protein Precipitates to Adsorb 

 Toxins 188 



c. Failure of Protein-Anti-Protein Precipitates to Adsorb Non- 

 Specific Colored Proteins 189 



d. Failure of Protein- Anti-Protein Precipitates to Adsorb Non- 

 Specific Proteins 190 



e. Failure of Agglutinated Bacteria to Adsorb Non-Specific 

 Proteins 191 



3. Effect of pH of Optimal Activity on the Nature and Extent of 



the Antigen-Antibody or Enzyme-Anti-Enzyme Combinations 192 



B. A Critical Consideration of the Antigen- Antibody Reactions in 

 Relation to the Inhibition of Enzymes by Specific Antibodies . 195 

 1 . An Analysis of the Reactions of the Active Groups of Proteins 



in Relation to Their Biological Specificities 195 



a. Studies on the Reactive Groups of Proteins 196 



b. Reaction of Formaldehyde with Proteins and Amino Acids 196 



c. Acylation of Proteins 198 



d. lodination of Proteins 200 



e. Reactions wdth Sulfhydryl and Disulfide Groups of Proteins 201 



