CONTENTS XV 



Page 

 2. Identity of the Nature of the Inhibition of Enzymes by Homol- 

 ogous Antibodies with Antigen-Antibody Reactions . 204 



C. Antibody Against Carbohydrases 213 



1. Antibody Against Emulsin 214 



a. Inhibition of the Amylase Activity of Emulsin Preparation 



by Anti-Emulsin Serum 215 



2. Specificities of Amylases 218 



a. Antibody Against Malt Amylase 220 



3. Invertase and Its Properties 223 



a. Antibody Against Invertase 224 



4. Enz)Tnatic Synthesis of Serologically and Physiologically Ac- 

 tive Polysaccharides 225 



5. Antibody Against Bacterial Polysaccharidases 230 



6. Antibody Against Crystalline Lysozyme 230 



D. Hyaluronidase or the Permeability Factor 231 



1. Discovery of the Permeability Factor 231 



2. Enz)Tnic Nature of the Permeability Factor and the Nature 



of the Substrate 233 



3. Mechanism of the Action of Hyaluronidase 237 



a. Quantitative Methods of Measuring Hyaluronidase Acti\aty 239 



4. Distribution and Physiological Significance of Hyaluronidase 



or the Permeability Factor 241 



a. Relation of Hyaluronidase Production to Virulence of Bac- 

 teria 241 



b. Relation of Capsular Polysaccharide and Hyaluronidase to 



the Virulence of Streptococcus and Pneumococcus . 244 



c. Hyaluronidase in Infected Tissue Extracts 245 



d. Effect of Hyaluronidase on Fertilization 246 



5. Non-Specific and Specific Inhibitions of Hyaluronidase 246 



a. Non-Specific Inhibition of Hyaluronidase 246 



b. Neutralization of Hyaluronidase or the Permeability Factor 



by Specific Immune Serum 247 



E. Antibody Against Proteolytic Enzymes 249 



1. Antibody Against a "Tr\^sin" Preparation 249 



a. Antitryptic Property of Guinea Pig Serum Immunized vdth 

 "Trypsin" 251 



b. The Toxic and Lethal Action of the Trypsin Preparation 252 



c. Neutralization of the Toxic Effects of Trypsin with Im- 

 mune Serum 253 



d. Antibody Against Papain 253 



e. Antibody Against the Proteolytic Activity' of Snake Venom 255 



f. Proteolytic Acti\aty of the Toxin of CI. Welchii . 256 

 2. Antibody Against Bacterial Proteinases 257 



