230 IMMUNO-CATALYSIS 



cent reducing sugar. They reacted specifically with anti-rabbit sera 

 prepared against these two organisms which had been grown in sucrose, 

 and failed, except with some of the type 20 antipneumococcal sera, to 

 react with other sera including those capable of reacting with dextran 

 polysaccharide. 



The purified levulan of Stre'ptococcus salivarius was serologically 

 reactive in 1 to 500,000, and that of Bacillus N9 in 1 to 2,000,000 

 dilution. Immunization of rabbits with suspensions of these bacteria 

 which had grown in sucrose media regularly yielded levulan reactive 

 immune sera, whereas immunizations with suspensions of the same 

 species of bacteria grown in glucose media regularly yielded antisera 

 which were entirely non-reactive with levulans although they did 

 agglutinate in high dilutions suspensions of the bacteria which had 

 been used for immunization. 



5. Antibody Against Bacterial Polysaccharidases 



Sickles and Shaw (1950) obtained antisera in the rabbits against 

 the polysaccharidases isolated from various strains of B. 'palustris. 

 These enzymes decomposed the capsular polysaccharides of pneumo- 

 cocci Types III and VII. The enzyme which specifically decomposed 

 Type III polysaccharide was neutralized by homologous anti-enzyme 

 immune sera and none of these antisera were specifically active against 

 Type VII polysaccharidase and vice versa. 



In mouse protection tests corresponding to the neutralization tests 

 in vitro, the results were comparable. The results showed that the neu- 

 tralizing activities of immune sera were directed specifically against 

 the polysaccharidases. 



6. Antibody Against Crystalline Lysozyme 



Lysozyme is an enzyme which lyzes Micrococcus lysodeikticus, and 

 Sarcina lutea. According to Meyer and Hahnel (1946) the substrate 

 is a mucopolysaccharide, which is rapidly depolymerized by lysozyme. 

 Optimal conditions for the antibacterial action of lysozyme on M. lyso- 

 deikticus is said to parallel in general the conditions for its depolymer- 

 izing action (Feiner, Meyer, and Steinberg, 1946), The polysaccharide 

 isolated from M. lysodeikticus is precipitable by antibacterial serum 

 and is one of the antigenic components of the organism. Feiner, et al. 



