ANTI-ENZYME IMMUNITY 



259 



sera. Rabbits immunized subcutaneously or intravenously witb filtered 

 culture fluid containing carbolic acid yielded sera of equal potency. 

 The rabbits were given injections every four to five days for two 

 months, and in some cases, for three months. 



Table XIII 



Inhibitory Action of Antisera on the Hydrolysis of Gelatin by 

 Enzyme Preparations 



Pyocyaneus Immune Serum -{-0.5 ml. of Enzyme Solution 



sign indicates absence of hydrolysis. 



-| — \- sign indicates hydrolysis. 



The results show that 0.025 ml. of immune serum completely in- 

 hibited the proteolytic activity of 0.5 ml. of the enzyme solution. In 

 contrast, normal sera showed no inhibitory action. 



The anti-enzyme (from B. siihtilis^ immune serum behaved simi- 

 larly, though somewhat less strongly. The antisera had no inhibitory 

 action on trypsin or other bacterial proteinases. B. siihtilis anti-serum 

 had no action on B. fyocyaneus and vice versa. 



h. Antibody Against Streptococcal Proteinase. According to Elliott 

 (1945) Group A Streptococcus ^pyogenes under certain conditions pro- 

 duce an extracellular proteinase which digests the type-specific M 



