ANTI-ENZYME IMMUNITY 



287 



Series D 



Results 



(a) 0. 1 ml. of unhealed rabbit serum ] 

 -|-0.6 ml. of heated immune guinea pig serum \ 

 -j-0.3 ml. of avian plasma ] 



(b) 0. 1 ml. of unhealed normal guinea pig serum ] 

 -|-0.6 ml. of heated immune guinea pig serum 

 -j-0.3 ml. of avian plasma 



(c) 0. 1 ml. of unhealed immune guinea pig serum ' 

 -{-0.6 ml. of heated immune guinea pig serum ' 

 -|-0.3 ml. of avian plasma 



Remains indefinitely liq- 

 uid 



Clots, though at a slower 

 rale 



Clotting complete within 

 90 minutes without 

 pencillia 



Series E 



(a) 0. 1 ml. of unhealed normal rabbit serum 

 -|-0.6 ml. of heated normal rabbit serum 

 -|-0.3 ml. of avian plasma 



(b) 0.1 ml. of unhealed normal guinea pig serum 

 -|-0.6 ml. of heated normal rabbit serum 

 -|-0.3 ml. of avian plasma 



(c) 0. 1 ml. of unhealed immune guinea pig serum 

 -|-0.6 ml. of healed normal rabbit serum 

 -j-0.3 ml. of avian plasma 



Clotting immediate 



Clotting immediate 



Clotting immediate 



was prepared by centrifuging oxalated rabbit blood. The oxalated 

 plasma was treated with a calculated amount of calcium chloride and 

 a dose of 5 ml. was then subcutaneously injected into guinea pigs. 

 The oxalated plasma thus treated with calcium chloride did not clot 

 until after a period of 10-12 minutes. Injections were carried out 

 before the lapse of this period. Using the above technique it was 

 possible to inject the animals with the fibrinogen mixed with fibrin 

 enzyme (thrombin) without causing clotting. 



The immunized guinea pigs were bled 12 days after the last injec- 

 tion. The immune serum was found to neutralize the thrombin ac- 

 tivity of fresh rabbit blood or serum. Non-clotting avian plasma with 

 high fibrinogen content was used as the substrate in inhibition or 

 neutralization experiments. 



The Avian Plasma. Bordet and Gengou found that avian plasma 

 clots very slowly if the bleeding is carried out without contaminating 



