394 



IMMUNO-CATALYSIS 



The inhibitory effect of the immune sera was tested using guaiacol 

 as substrate. One ml. of 0.1 per cent guaiacol solution was treated 

 with 2 ml. of buffer solution, 0.1 ml. of laccase solution and 0.1 ml. 

 of rabbit anti-laccase serum; the final volume was made up to 10 ml. 

 After the reaction mixture had been allowed to react for one hour, the 

 color was measured colorimetrically. The following results were 

 obtained : 



Table XIX 



In another experiment, pyrogallol was used as substrate. The sys- 

 tems contained 0.1 g. of pyrogallol, 2 ml. of buffer solution, 0.2 ml. 

 of serum, 0.1 ml. of laccase solution; the volume was made up to 10 

 ml. with water. Measurement was made after 24 hours. Purpuragallin 

 formed was filtered, dissolved in sulfuric acid and titrated with 0.1 N 

 potassium permanganate solution. The follov\dng results were ob- 

 tained: 



Table XX 



* Contained buffer. 



t Contained no buffer. 



The authors stated that the above results were corroborated re- 

 peatedly by the data of numerous unpublished experiments. They 

 did not find a difference in the buffering capacity of immune and 

 normal serum. To eliminate any possibility that a difference in the 

 physical properties of the two sera might be responsible for the in- 



