130 



II. DIGESTION AND ABSORPTION OF PATS 



Sullmann and Wilbrandt 616 employed a technic on rabbits whereby in- 

 testinal lymph can be collected separately from systemic lymph. The 

 large lymphatic vessels to the cisterna chyli can easily be found, especially 

 if the left innominate vein is ligated and if the animal has previously been 

 fed fat. If the lymphatic vessels are then cut with fine scissors, the lymph 

 will collect in a little lake, and this can be continually pipetted off over a 

 prolonged period. In their later work, Frolicher and Sullmann 136 modified 



Fig. 12. Appearance of fresh blood under the high-power dark-held microscope 

 to demonstrate the chylomicrons. The field at the left represents the appearance 

 of blood obtained after a 16-hour to 24-hour fast. The field pictured at the right 

 is for blood obtained 3 to 4 hours following a generous fat meal. 617 



the technic by omitting ligation of the innominate vein; thus, the lymph 

 from other parts of the body could flow in the usual channels and not be 

 shunted back into the intestinal lymphatics. This was considered to give 

 a much purer "enteric lymph." A procedure for the separate collection 

 of intestinal and thoracic duct lymph in the rat has been devised by Boll- 

 man and associates. 618 



b. Chylomicron Method. This procedure was first described in the 

 classical paper of Gage and Fish 617 published in 1924. These investigators 



616 H. Sullmann and W. Wilbrandt, Biochem. Z., 270, 52-62 (1934). 

 817 S. H. Gage and P. A. Fish, Am. J. Anat., 84, 1-86 (1924). 



fil8 J. L. Bollman, J. C. Cain, and J. H. Grindlay, ./. Lab. Clin. Med., 38, 1349-1352 

 (1948). 



