ABSORPTION AND TRANSPORT OF FATS 149 



iodoacetate or phlorhizin was given. 701 Since these substances inhibit the 

 phosphorylation of hexoses, it was assumed that they behaved in a similar 

 manner toward the fatty acids. In the absence of phosphorylation of these 

 compounds, a resyn thesis of neutral fat did not take place, and the absorp- 

 tion of the fatty acids was effectively blocked. Verzar and Laszt 705 ' 706 

 have likewise found that fat absorption is slowed down in adrenalecto- 

 mized animals. This is interpreted to mean that the mechanism control- 

 ling phosphorylation is upset. The livers were not poisoned by phosphorus 

 after extirpation of the adrenals, and fat could not be removed from the 

 fatty livers under such conditions. Laszt and Verzar 707 attribute this to a 

 failure of the phosphorylating mechanism to function. All these conditions 

 were cleared up by adrenocortical extract. 708 



Bavetta et al. 709 confirmed the findings of the Verzar group by establish- 

 ing the fact that fat absorption is decreased in rats following removal of the 

 adrenal cortex, and that the deficiency is cleared up by the prefeeding of 

 adrenocortical extract. The failure of Barnes et al. lw to observe a de- 

 crease in the rate of fat absorption following adrenalectomy has been as- 

 cribed to the fact that old animals were used, in which case the absence 

 of the adrenal cortex is less critical than in young animals. 709 



In addition to the results of the Verzar group, other evidence of the im- 

 portance of the phosphorylating mechanism has continued to accumulate. 

 Thus, Artom and Peretti 711 found iodized fatty acids in the phospholipid 

 fraction of intestinal mucosa after feeding large doses of iodized fat to rats. 

 Similar results were obtained with cats by Sinclair and Smith, 712 who found 

 that the intestinal phospholipids contained as much as 35% of elaidic acid 

 after the unnatural fat, trielaidin, had been fed. Perlman et al. in found 

 that larger amounts of P 32 were present in the intestinal phospholipid 

 when the radioactive phosphate was given with cod-liver oil than when it 

 was administered without fat. 



According to Favarger et al., 7u the site of the most active synthesis of 



705 F. Verzar and L. Laszt, Biochem. Z., 276, 11-16 (1935). 



706 F. Verzar and L. Laszt, Biochem. Z., 278, 396-400 (1935). 



707 L. Laszt and F. Verzar, Biochem. Z., 285, 356-367 (1936). 



708 L. Laszt and F. Verzar, Biochem. Z., 288, 351-355 (1936). 



709 L. A. Bavetta, L. Hallman, H. J. Deuel, Jr., and P. O. Greeley, Am. J. Physiol., 

 184, 619-622 (1941). 



710 R. H. Barnes, E. S. Miller, and G. O. Burr., J. Biol. Chem., IJfi, 241-246 (1941 ). 



711 C. Artom and G. Peretti, Arch, intern, physiol., 42, 61-94 (1935). 



712 R. G. Sinclair and C. Smith, J. Biol. Chem., 121, 361-372 (1937). 



713 1. Perlman, S. Ruben, and I. L. Chaikoff, J. Biol. Chem., 122, 169-182 (1937-1938). 

 714 P. Favarger, R. A. Collet, and P. Veraguth, Bull. soc. chim. biol., 31, 384-388 

 (1949). 



