EDWARD S. KLINE 



159 



TABLE 5 

 Fractional separation of succinoxidase inhibitor from Hydra (from ref. 6) 



Lyophylized homogenate 



Extracted with n-butvl alcohol 



Soluble (inactive) 



Soluble (inactive) 



Residue ( active ) 

 Extracted with acetone 



Residue (active) 

 Extracted with ether 



Soluble (inactive) Residue (active) 



Extracted with 0.02 M phosphate buffer, pH 8 



Phosphate soluble 



( about 92% of the activity ) ' 



pH adjusted to 3.9 



Residue 



( about 8% of the activity ) 



Supernatant 



Dialized against 0.02 M phosphate buffer, pH 8 



Fraction A 

 ( about 75% of activity in "phosphate soluble" fraction ) 

 Centrifuged in Spinco preparative ultracentrifuge 



Residue— discarded 



Supernatant— purified inhibitor 

 (Almost all of activity in Fraction A) 



'Sample dialyzed before activity determination. 



Residue— discarded 



