EDWARD S. KLINE 



161 



.04 



.08 



.12 .16 .20 .24 .28 



ML. OF PURIFIED INHIBITOR 



32 



.36 



Fig. 4. The inhibitory effect of the purified inhibitor on the succinoxidose 

 activity of mouse liver homogenate. In both Curve A and Curve B, the flasks 

 contain 0.1 ml. of liver homogenate (5 mg. of wet tissue per flask) and 

 the purified inhibitor (2.9 /;g. of protein nitrogen per ml. of inhibitor). Total 

 volume in all flasks: 3.1 ml. Each curve represents separate experiments (6). 



TABLE 6 

 Effect of the purified inhibitor on succinic dedydrogenase 



Material 



Inhibitor to Succinic dehydrogenase Inhibition 

 tissue ratio activity 



Hg.: mg. Q02 (wet weight) % 



Mouse liver homogenate 

 Mouse liver homogenate 

 plus purified inhibitor 

 ( 100 ^g. protein N ) 



2.5 : 1 



4.0 



3.3 



17.5 



Each vessel contained 40 mg. of aqueous liver homogenate in final volume 

 of 2.9 ml. 



