LI AND LENHOFF 



447 



-1 120 



Isf feeding 2nd feeding 3rd feeding 



DAYS BUDS ARE GROWING 



100 



80 

 60 

 40 



O 



en 

 20 Q. 



Fig. 6. DNA, RNA, and protein content of buds during growth to small 

 bud stage Hydra. 



owing to an increase in protein, the bud's DNA/protein ratio dimin- 

 ished to that of the parent ( Fig. 6 ) . These experiments suggest that 

 in bud cells the amount of nuclear material is high relative to the 

 cytoplasm, and that subsequent growth of the detached bud 

 involves an increase in cytoplasmic components rather than mitosis. 



REFERENCES 



1. Burton, K. 1956. A study of the conditions and meclianism of diphenylamine 



reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. 

 }. 62: 315-323. 



2. Li, Y.-Y. F. and H. M. Lenhoff. 1960. Nucleic acid patterns of Hydra budding 



in synchrony. Anat. Rec. 137: 376. 



3. LooMis, W. F. and H. M. Lenhoff. 1956. Growth and sexual differentiation of 



hydra in mass culture. /. Exp. Zool. 132: 555-574. 



4. LowRY, O. H., N. J. RosEBROUGH, A. L. Farr and R. J. Randall. 1951. Protein 



measurement with the Folin phenol reagent. /. Biol. Chem. 193: 265-275. 



5. Schneider, W. C. 1957. Determination of nucleic acids in tissues by pentose 



analysis. Methods in Enzymologij 3: 680-691. Academic Press, Inc., New 

 York. 



