CONTENTS 

 INTRODUCTION 



I, Historical 1 



The Phenomenon of Bacteriophagy 1 



Bacterioclysis: The Twort Phenomenon 13 



II. Terminology and Technic 18 



Terminology 18 



Technical Procedures 21 



Ultrafiltration 24 



Preparation of ultrafilters 26 



Assembling the ultrafilter 29 



Denitrification 30 



Sterilization 32 



PART I. THE PHENOMENON OF BACTERIOPHAGY 

 Chapter I 



BACTERIOPHAGY IN A FLUID MEDIUM 



1. Isolation of the Bacteriophagous Principle 37 



Ubiquity of the bacteriophage 37 



Methods of isolation 38 



Technic of isolation 39 



2. Serial Action 40 



3. Environmental Conditions Favoring Bacteriophagy 41 



General conditions 41 



Reaction of the medium 43 



4. Effect of the Condition of the Bacterium 46 



5. Effects of the Relative Concentrations of Bacteriophage and Bacteria.. 49 



Limits of bacteriophagy 51 



Limits of activity of the bacteriophage principle 55 



6. Influence of Physical Conditions 58 



Action of heat 58 



Pressure conditions 63 



Viscosity of the medium 63 



7. Effect of the Chemical Conditions of the Medium 65 



Colloids 65 



Dyestuffs 67 



Electrolytes 68 



Sugars 70 



Salts 70 



Body fluids and other substances 71 



Antiseptics 71 



IX 



j^3l 



