BACTERIOPHAGE AS AN ANTIGEN 



399 



materials were added as indicated in table 50, which also records the 

 results obtained. 



From these experiments we find that the ''antibacteriophage agent" 

 contained in 4 drops of the antibacteriophage-coli serum is neutralized 

 by 12 drops of the "anti-antiserum." This "anti-anti" property re- 

 sisted heating at 56°C. for 30 minutes. 



Fabry and van Beneden concluded that the injection of an anti- 

 bacteriophagic serum (rabbit) into a different and unrelated animal 



TABLE 50 

 Each of the twelve tubes contains 10 cc. of bouillon implanted with 1 drop of 

 B. coli culture. 



* In the course of a number of experiments they observed, in some instances, 

 that the serum of a normal rabbit inhibited the action of the Coli-bacteriophage, 

 while the sera of other rabbits were without inhibitory action. 



species (dog) led to the formation within the dog of "anti-anti" prop- 

 erties capable of neutrahzing in vitro the antibacteriophagic serum. 

 In reality, this is not a true "anti-anti" action, and this is clearly shown 

 by the fact that it is impossible to produce such a serum in animals of 

 the same species or in related species. Certainly the effect observed 

 is referable to a precipitation of the globulins. Dehne and Hamburger* 



* Dehne R., and Hamburger, F.— Experimenteluntersuchungen liber die 

 Folgen parenteraler Einverleibung von Pferdserum. Wien. klin. Wchnschr., 

 1904, 17, 807. 



