554 THE BACTERIOPHAGE AND ITS BEHAVIOR 



fever. The general condition was poor; the patient was emaciated. 

 Up to the present time the disease had run a cycHc course, acute at- 

 tacks following periods of remission. 



The present attack had persisted for several weeks. Treatment of 

 various kinds,— application of heat to the pelvis, urotropin, — had been 

 instituted, but no improvement resulted. Bacteriological examination 

 showed the urine to be virtually a culture of B. coli, and this B. coli 

 was susceptible to a race of the bacteriophage maintained in the labora- 

 tory. Two cubic centimeters of this bacteriophage suspension were 

 injected subcutaneously in the thigh. The next day there was a slight 

 redness and swelhng and a Httle pain. A second injection of 2 cc. was 

 given on the following day. A local and not painful reaction followed. 



The day following the second injection the pain associated with urina- 

 tion had disappeared, and urination was less frequent. During the 

 following days the need for urination became less and less frequent. - 

 On the 5th day, bacteriological examination showed that the urine was 

 sterile. Two months later the patient had gained 1 1 pounds, the general 

 condition was excellent, urination was normal and recovery was com- 

 plete and permanent. 



In brief, this was a case of chronic cystitis due to B. coli. Medical 

 treatment had failed. Chnical and bacteriological cure occurred, at the 

 expense of a very shght local reaction, within 7 days after 2 injections 

 of the bacteriophage. 



Case 2. Mrs. F. A primipara in the 5th month of pregnancy. 

 An acute case of pyelonephritis on the right side. Eight days after the 

 onset of the attack the temperature had fallen somewhat, but the kidney 

 remained large, very sensitive, and the urine was cloudy. Bacteriologi- 

 cal examination showed B. coli to be present ; the B. coli was susceptible. 



The patient was given a subcutaneous injection of 1 cc. of Coli- 

 bacteriophage and it was instilled into the bladder twice. Improve- 

 ment was very prompt. The patient got up a few days after the in- 

 jection and at this time the urine was clear and culturally sterile. 



Case 3. A female child, 1 year old. The general condition was poor. 

 There was a B. coli pyelitis. The treatment was: 



On April 19, a subcutaneous injection of 0.75 cc. 



On April 22, a second injection of the same bacteriophage. 



This same day the urine was clear. Bacteriological examination 

 on April 25 showed the urine to be sterile. Recovery was complete. 



Case 4. An infant with a recent acute pyehtis. The urine yielded 

 an abundance of B. coli; the strain was susceptible. Two injections 

 of 0.5 cc. of a Coli-bacteriophage were given, with a 48-hour interval. 



