556 THE BACTERIOPHAGE AND ITS BEHAVIOR 



I have treated a dozen cases of simple cystitis or pyelonephritis, in 

 which I had previously shown that the pathogenic B. coli was susceptible 

 to one of my races of bacteriophage. In the treatment of all of these 

 cases the suspension of the bacteriophage which I used was prepared by 

 the complete bacteriophagy of a suspension of B. coli containing 250 

 million bacilli per cubic centimeter. In some of the cases the bacterio- 

 phage used was developed at the expense of the colon strain isolated 

 from the patient, in others, it was developed at the expense of a labora- 

 tory strain. I was unable to detect any difference in the therapeutic 

 effects; the single condition essential to a therapeutic success being 

 that the bacteriophage inoculated be active for the pathogenic bacillus 

 of the patient. 



I have always apphed two injections of a maximum of 2 cc, at a 24 

 hour, or at a maximum, a 48 hour interval, and I have given at the same 

 time bladder instillations with a 1 : 10 dilution in saline of the bacterio- 

 phage suspension. 



We have seen that repeated injections of the bacteriophage may lead 

 to the development of the state of antiphylaxis. Hence, the injections 

 should not be repeated indefinitely. Furthermore, experiment shows 

 that two are sufficient, for if beneficial results are not obtained with 

 them, it means that the race employed is devoid of action. As for the 

 instillations, they may be repeated as many times as seem advisable, 

 for they do not lead to antiphylaxis. Ingestions are also harmless in 

 this respect as I have shown. To test this last point, I ingested daily 

 throughout a period of a month, 10 cc. of bacteriophage suspensions 

 virulent for B. coli, B. dysenteriae, and for B. paratyphosus A and B. 

 No harmful effects followed. 



But to return to the treatment of B. coli infections of the urinary 

 tract. In the 12 cases which I have treated, being assured always in 

 advance that the bacterium was susceptible to the bacteriophage, 

 clinical and bacteriological cure was obtained within 5 days of the last 

 injection, with the exception of the first case treated. Here is the 

 history of this patient. 



A woman, aged 30 years, had suffered from a cystitis for 7 years. The 

 patient had been under the care of several speciaHsts who had apphed 

 various forms of treatment, including auto-vaccines at two different 

 times, some 30 injections being given in each series. In neither in- 

 stance could any beneficial effect be observed. 



The B. coli isolated from the urine proved to be very susceptible and 

 I prepared material for use in treatment by the bacteriophagy of a 



