574 THE BACTERIOPHAGE AND ITS BEHAVIOR 



the gland I injected into the bubo 1 cc. of a suspension of bacteriophage 

 virulent for B. pestis. This treatment was given, therefore, about 60 

 hours after the onset of the disease. 



On the 28th the bubo was much less painful, and there was a slight 

 fall in the temperature. The general condition of the patient appeared 

 somewhat better, but from the manifest disturbances in the heart 

 action there seemed to be a severe toxemia. Camphorated oil was 

 injected and sparteine was administered. 



On the 29 th the temperature again rose and the patient became 

 delirious. The tongue was dry and brownish. The condition ady- 

 namic. The pulse was weak, 110 beats per minute, with a pause between 

 every three or four beats. At 4 o'clock in the afternoon I gave a second 

 injection into the bubo, 1.5 cc. of the bacteriophage suspension being 

 given. 



On the morning of the 30th the condition of the patient was much 

 better. While on the previous evening the case had seemed hopeless, 

 in the morning the patient was stronger, the temperature had fallen, 

 the pulse was 92, and the irregularities occurred about every 15 beats. 

 By evening the improvement was still more marked. The pulse was 

 76 and the pauses in the beats occurred only about once in 25 beats. 



On the 31st the patient was bright and the general condition was good. 

 Pulse 80, of good quality and regular. 



Throughout the following days the improvement continued. 



On August 2nd, at about 5 p.m., the patient complained of a pain in 

 the left groin. On the 3rd the left crural gland was swollen to the size 

 of a small nut. The gland was aspirated and a few drops of pus were 

 obtained. Direct examination showed only polymorphonuclear leuco- 

 cytes with a few coccobacilli with bipolar staining. Seedings of this 

 material in broth and on agar remained sterile. A guinea pig inoculated 

 with the material showed no evidence of infection up to 8 days later. 

 The bouillon which had been implanted and which had remained without 

 growth was seeded after 48 hours with a B. jyestis culture. Bacteri- 

 ophagy was complete in 8 hours. It is of interest to note that the two 

 inoculations of bacteriophage had been made into the right bubo, and 

 that nothing had been injected into the one on the left side. 



On the 8th of August this bubo had in large part been absorbed. 

 Chart 25 gives the curve showing the fluctuations in the temperature 

 of this patient. 



In 1921 I published the observations on 5 patients affected with 

 bacillary dysentery which I had treated by the ingestion of the bacterio- 



