NUCLEAR PROTEIN SYNTHESIS 



273 



•| 100 



£ 



^ 0-80 



0'60 



E 0-40 



0-20 



Alanine-1-C 



IO°C 



6 7 8 



pH of nuclear suspension 



Fig. 6. The pH dependence of the nuclear alanine transport reaction. The 

 rate of amino acid transport (in m/^moles of L-alanine per 10 min. at 10°) is plotted 

 against the pH of the incubation medium. 



e 



^ 400 



z 



E 



300 



u 200 



T, 100 



Alanine-1-C 

 pH 6-65 



10 20 30 40 50 60 

 Temperature ( C) 



Fig. 7. The temperature dependence of the nuclear alanine transport reaction. 

 The rate of amino acid transport (in m/Limoles of L-alanine per 10 min. at pH 6 • 75) 

 is plotted against the temperature of incubation. 



T 



