T I I I — I — I — I — I — I — r 



serum albumin 



PARTITION OF MACROMOLECULES IN AQUEOUS TWO-PHASE SYSTEMS 



2.0 



f.a 



1.6 



/.^ 



1.2 



o.e 



0.6 



0.2 



A I I \ L 



37 



"S (V o 



tT^2>0- 



2 3 ^ 5 6 7 e 9 10 n 12 

 pH 

 Fig. 3. Partition coefficient, A', of serum albumin as a function of pH in a 

 dextran-methylcellulose system. From ref. [3]. 

 100 



ao 

 7o 

 60 

 so 

 <o 



20 



15 



NaCI 



Fig. 4. Partition coefficient, K, of a number of proteins in a dextran-poly- 

 ethylene glycol system with 0005 M KH0PO4 and increasing concentrations of 

 NaCl ; the latter is expressed as moles NaCl added to i kg of phase system. For 

 experimental details see ref. [3]. 



