114 



E. S. CANELLAKIS AND EDWARD HERBERT 



TABLE I 

 Summary of Purification Procedure 



* The specific activity unit is expressed as /x//moles of [^*C]-AMP incorporated 

 into S-RNA in the presence of i mg. protein in 6 min. at 25°. 



Buffer cone 



Volu 



280 ml. 



Fig. I. Stepwise procedure for elution of ribonucleoproteins from an hydroxy- 

 apatite column. The width of the steps in the chromatogram represents the volume 

 of eluate collected in each fraction. These volumes are as follows in the order of 

 potassium phosphate buffer pH 7 ■ 2 concentrations listed under the chromatogram 

 (0-05 M, o-o8 M, and 0-12 m); 20 ml., 10 ml., and 25 ml. Column dimensions 

 2 '5 cm. X 20 cm. 



