STUDIES ON MECHANISM OF SYNTHESIS OF SOLUBLE RIBONUCLEIC ACID II5 



Buffer 



Volume (ml ) Conc- 

 Mixer 300 OOIM 



Reservoir 



300 0-25 M 



• Ribonucleoproteins 



2-5M buffer 

 (HEME color) 



20 30 40 50 60 70 

 Tube number 



Fig. 2. Linear gradient procedure for elution of ribonucleoproteins from an 

 hydroxylapatite column with potassium phosphate buffer pH 7-2. The volume of 

 eluate collected per tube is i o ml. The 2 • 5 M buffer is added directly to the column 

 and collected in 10 ml. portions. Column dimensions 2-5 cm. x 20 cm. 



2 1 



1-2 

 06 



200 

 100 

 08 



3 

 04 







■^ 



"RNA" 

 component 



"Protein" 

 component 



Buffer cone Eff 

 NaCI cone. 

 Volume (ml ) lO 



OIM 

 

 15 



T" 



OOSM 







10 



007SM 

 

 15 



II25M 

 

 15 



02M 



02M 



10 



J r 



2M j 



5M 



12 



Fig. 3. Stepwise elution and separation of protein and RNA component on 

 a DEIAE column, with potassium phosphate buffer pH 72 and sodium chloride. 

 Column dimensions i -5 cm. x 2 cm. 



We have termed these S-RNA-protein fractions "ribonucleoproteins" 

 for the following reasons: (a) the protein and the S-RNA fractionate 

 together during an extensive purification process, (b) the protein and the 



