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Discussion 



DoRF.MAN : I would like to mention one thing that may have some connection, 

 somewhat far fetched, relating to the biosynthesis of the membrane, and that is in 

 the case of our study of the synthesis of polyuronic acid in bacteria. We have been 

 able to localize the enzyme of the final polymerization in the cells of the protoplast 

 membrane, which can be prepared pure. There is no RX.A. left in them and thus 

 this membrane is serving a function similar to the endoplasmic reticulum in the 

 cells that Dr. Holter suggested. I just wonder whether in this question of transport 

 if it isn't only the final polymerization of the macromolecules that is occurring 

 at this anatomically localized site because all the other enzymic activities required 

 to forni the viridine nucleotides are all perfectly soluble and removed. It is only the 

 final polymerizing reaction that is localized to these membranes. 



Campbell: I would like to ask about the possibility o j-'^hether the lysosomes 

 are derived from the pinocytotic vesicles. I think it is shown from the work of 



