200 GOTTWALT CHRISTIAN HIRSCH 



vesicles and Golgi vacuoles steadily growing larger and denser. Finally the 

 zymogen granules are surrounded by a special membrane, containing a 

 large amount of packed enzymes. This work of condensing seems to be the 

 general function of the Golgi bodies [67, 69, 71]. 



5. What is the bridge between the intracisternal granules on one hand 

 and the condensation inside the Golgi bodies on the other ? There must be 

 a connection which carries the enzymes from the endoplasmic reticulum 

 to the Golgi field. 



There are two possibilities. First, that the intracisternal granules are 

 dissolved in the lumen of the endoplasmic reticulum and their parts 



Fig. 2. A living pancreas cell of the acinus with good blood circulation. 

 Pilocarpine stimulation 15.40 hr. First illustration at 16.40 is of entire cell, then 

 observation of a single constellation of three granules. The mitochondrion here 

 observed moves in a snakelike manner but was not further observed. The move- 

 ment of the three granules between 17.00 and 20.10 as they moved to the Golgi- 

 field, where they disappeared. It is probable that this type of granule is identical 

 with the intracisternal granules of G. E. Palade. (From G. C. Hirsch [66].) 



(enzymes ?) are brought to the apex by currents in the canals. Secondly, 

 that the intracisternal granules wander undissolved in the canals to the 

 Golgi field. Both possibilities are probable in certain cases, but the dis- 

 solving of these granules on the cell base has until now not been seen in 

 the living cell. The intracisternal granules are large and can be seen also 

 in the light microscope. The wandering of these granules has been observed 

 many times (Fig. 2). 



This wandering was discovered by Hirsch. In the living pancreas 

 after pilocarpine stimulation, small granules are produced on the cell 

 base in the ergastoplasm. They have a diameter of approx. 0-3 to 0-4 jjl 

 (Hirsch's specification of 0-03 [x is a printing error). The endoplasmic 

 reticulum at that time was not to be seen, but the mitochondria embedded 

 in the reticulum were. Because of this Hirsch made the mistake that the 



