2l6 



PETER PERLMANN AND WINFIELD S. MORGAN 



isotopic amino acids; for details see [lo]. The Ouchterlony reactions of all 

 four agar plates were practically identical and very similar to those shown 

 in Fig. 2(rt). Figure 3 demonstrates again that the radioactive label first 

 appears in the microsomal serum proteins, reacting with an antiserum 

 against rat serum, and in some additional microsomal antigens, reacting 



a-cs 



a-m 



i-cs 



a-m 



a-cs 



a-m! 



a-cs 



a-m 



EOTA* I EDTA*^^ 



fi-pHS a~cs, ^-PHJ 



(«) 



EDT, 



CS' 



r 



a-rs a-m? 



L' 



a-pHs a-cs 



t") 



■la-rs 



(b) 



4^"i 



a-pH5 



a-rs a-m ' a-rs 



EDTA* 



f > 



a-pH5 



a-cs 



a.pH5 



\ 



a-rs a-m 



|cs'^ 



a-rs 



(0 



Fig. 3 



id) 



