244 PHILIP SIEKEVITZ 



TABLE III 



Effect of Spermine and of Mg + + on Release of RNA and 

 Protein from RNP Particles 



Incubation conditions are given in the text. 



Exp. 

 No. 



Additions 



Left in RNP particles after 



spinning incubated particles for 



90 min. at 105 000 x g 



RNA 



4 None 



Spermine (10"* m) 



Spermine (10^ m) plus Mg + + (io~^ m) 



Protein 



RNA/ 

 Protein 



I None 229 341 0-67 



Spermine ( I o"* m) 209 278 0-75 



Spermine (lO"* m) plus Mg + + (lo"^ m) 273 319 0-85 



6 12 18 24 30 36 42 48 54 60 



GTP (xIO'^'m) 



Fig. I . Effect of GTP concentration on the bound radioactive spermine, on the 

 RNA, and on the RNA/protein ratio of the RNP particles. Methods are given in 

 the text. 



addition of Mg + + along with the spermine results in a very weak Mg + +- 

 spermine complex and hence has no effect on the salts formed between 

 the spermine and the phosphate groups. Since the spermine would appear 



