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PHILIP SIEKEVITZ 



TABLE V 



Effect of GTP and of Mg + + on the Release of Radioactive Spermine, and 

 OF Protein and RNA from RNP Particles 



The RNP particles were labelled with radioactive spermine as described in 

 the text. These particles were then re-suspended and re-incubated at 35° for 30 

 min. under the conditions given in the text and with additions given below. 

 After re-incubation, the particles were spun at 105 000 x g for 90 min., and 

 radioactivity, protein, and RNA determined as described in the text. 



that the ratio, /xmoles Mg + +/io /xmoles RNA-P, in the RNP particles 

 was approximately one. In Table IV it can be seen that this ratio for 

 spermine varied from 0-29 to i -49 in the various experiments; or, if put 

 in another way, from o -6 to 3 -o /xmoles primary amine /lo jumoles RNA-P, 

 or from 1-2 to 6-o /xmoles total amine group /lo /imoles RNA-P. It can 

 be noticed that these experiments of Table IV fall into three classes ; one 



