MUCOPOLYSACCHARIDES OF CONNECTIVE TISSUE 



TABLE II 



The Separation of Acid Mucopolysaccharides from 

 AN Extract of Rat Skin* 



335 



Flask No. 



Solvent 



Volume 



Uronic acid 



1 Supernatant 



2 003 M XaCl in oiO„ CPC 



3 0-4 M NaCl in o-if-o CPC 



4 



5 



6 



7 

 8 



9 I • 2 M NaCl in o • I "^'o CPC 



10 

 II 

 12 

 13 



14 2-1 M NaCl 



IS 

 16 



754 



* The uronic acid content was 5 ■ 89 mg. in a volume of 9 ■ 5 ml. To this were 

 added 0-38 ml. of i ivi NaCl and 0-32 ml. of a 10*^0 solution of CPC, bringing the 

 total volume to 10 -2 ml. The mixture was incubated for i hr. at 37". Approximately 

 200 mg. of Celite were added prior to centrifugation as described in the text, o • 2 

 to I -o ml. aliquots were removed from each flask for uronic acid determinations. 

 The recovery was 5-97 mg. uronic acid or ioi°o- 



was achieved. The procedure has now been successfully adapted to analysis 

 of human skin, human aorta, human lung, and basement membranes of 

 dog and human kidney. 



Table IV illustrates a series of analyses on rat skin [50]. A surprisingly 

 large amount of heparin w^as found. Variation of polysaccharide content 

 with age was noted. Particularly striking was the decrease in concentration 

 of heparin beyond 44 days. Little or no heparin was found in hog, guinea- 

 pig, rabbit, foetal calf, camel or human skin. The physiological significance 



