SEPARATION OF OLIGOSACCHARIDES WITH GEL FILTRATION 349 



Discussion 



Hestrin : Would it be possible with the help of this method to separate linear 

 from branched oligosaccharides when they have the same molecular weight ? 



Flodin : It might be possible. There is, however, no experimental evidence as 

 yet. 



Smith: Does this maximum molecular weight hold for proteins also ? 



Flodix : Roughly. The size is probably the determining factor in the separa- 

 tions and therefore the molecular weight, though more convenient to use, is an 

 inadequate measure. 



Smith: If you have a molecule which is the size of, say, 100 A could you do 

 anything with that ? 



Flodin : Molecules larger than about 50 000 in molecular weight can be 

 separated from smaller ones on the loosest gels. Even haemoglobin, however, is 

 somewhat retarded in relation to gamma globulin and serum albumin. 



