20 CONTENTS 



PAGE 



Manifestations of Tertiary Stage of Syphilis; Wassermann and 

 Bruck Modification of the Bordet-Gengou Technique for Bacterial 

 Complement Fixation, 423; Total Volume; Bacterial Antigens; 

 Complement; Immune or Patient's Serum; Ked Cell Suspension; 

 Hemolysin; The Application of Complement Fixation to the Diag- 

 nosis of Syphilis, 424; Controls Employed in the Original Wasser- 

 mann Technique, 425; Importance of Citron's Work; First Stage 

 of the Development of the Wassermann Eeaction; Second Stage Be- 

 gan With the Use of Alcoholic Extracts of Guinea Pig Heart, 426; 

 Third Stage Introduced by Cholesterinized Antigen; Fourth Stage: 

 Introduction of Improved Antigen, 427; Fifth Stage: Standard- 

 ization of Wassermann Reaction by Kolmer; Kolmer Test, 428; 

 Titration of Antigen, 429 ; Kilduff e 's Ten Basic Principles in Sero- 

 logical Diagnosis, 431 ; The Provocative Wassermann Reaction ; 

 Wassermann-Fast Cases, 432 ; Effect of Malarial and Diathermy 

 Treatment; Immunity to Syphilis, 4.'^? ; Nonspecific Wassermann 

 Reactions, 434 ; Mechanism of the Wassermann Reaction, 435. 



CHAPTER XXIV 



Precipitin Tests in Syphilis _____________ 444 



Introduction; Historical Development; Introduction of Cholesterol, 

 444; Simplicity of Flocculation Method; Status of Flocculation 

 Tests; Principle of Flocculation Tests, 445; Role of Ingredients, 

 446; Antigens; Synergistic Substances, 447; Role of Cholesterol; 

 Water, 448; Electrolytes; Alcohol, 449; Ratio of Components; 

 Concentration of Lipoids; Cholesterol-Lipoid Ratio, 450; Alcohol- 

 Water Ratio; Miscellaneous Factors; Finished Antigen, 451; Choice 

 of Test; Kahn Precipitation Reaction; Salt Titration, 452; Stand- 

 ardization of Antigen; Inactivation ; Shaking, 453; Time of Read- 

 ing Results; Controls; Choice of Kahn Test, 455; Presumptive 

 Test; Quantitative Test; Spinal Fluid Procedure; Application of 

 Quantitative Tests, 456; Kahn's Verification Test; Description of 

 Kline Test, 457; Sensitivity; Agreement With Other Tests; Tech- 

 nique of Tests; Preparation of Antigen; Reading Test, 458; Elimi- 

 nation Test; Use of Elimination Test; Sources of Error, 460; Prin- 

 ciple of the Meinicke Reaction; Specificity; Antigen; Mixing of 

 Antigen, 461; Serum; Technique of the Test; Interpretation of 

 Results, 462; Hinton Test; Glycerinated Indicator Solution; Tech- 

 nique of Test, 463; Citochol Reaction; Antigen; Serum, 464; Test; 

 Bruck 's Nitric Acid Reaction; Formol Reaction; Sachs-Georgi Re- 

 action; Vernes; Eagle Flocculation Test, 465; Rosenthal Test; 

 Mazzini Microflocculation Test; Importance of Using Both Com- 

 plement Fixation and Precipitation Tests; Hypersensitive Tests of 

 Doubtful Value, 466. 



