J> 1-. 



> 



1, 



^ W \-J 

 ABC 



F 



Plate V. — OuuiixAL Bordet-Gengou Bacterial Complement Fixation Test 



A B C D E F 



Complement 0.2 p.e. 0.2 c.c. 0.2 c.c. 0.2 c.c. 



Antigen 0.4 c.c. 0.4 ex. 0.4 e.e. 0.4 c.c. 



Antipestis serum (heated) 1.2 e.e. 1.2 cc. 1.2 c.e. 



Normal hor.se serum ( heated) 1.2 e.e. 1.2 c.c. 1.2 c.c. 



In<'ubate for fifteen to twenty hours at room temperature and add 0.2 c.c. sensi- 

 tized led cells and incubate at 37° C. for one liour. 



