BACTERIAL COMPLEMENT KLXATION" TECHNIQUE 417 



14. The method of antigen preparation depends to some extent 

 upon the organism used. It is suggested that the Kolmer or 

 New York City or New York State Board of Health methods 

 be used. For purposes of instruction defatted antigens are 

 recommended. 



15. An antigenic unit is the least amount of antigen or 0.5 e.c. 

 of the highest dilution of antigen that gives complete 

 (+ + + +) fixation of 2 full units of complement in the pres- 

 ence of 0.1 c.c. of known positive human serum. 



16. A satisfactory antigen must not be hemolytic or anticom- 

 plementary when 10 antigen units are present in the tubes. 



17. All immune, positive, negative, and patient's sera are to be 

 inactivated at 56° C. in a water bath for fifteen or twenty 

 minutes for the quantitative test and thirty minutes for the 

 simplified complement fixation test. 



References 



Bonlet, J., and Gengou, O. : Sur 1 'existence des substance.s sen.sibilisatrices 

 dans la plupart des serums antimicrobiens, Ann. de I'Inst. Pasteur 

 15: 289, 1901. 



Craig, Charles F. : Observations Upon the Hemolytic, Cytolytic and Com- 

 plement-Binding Properties of Extract.* of Endamebic Histolytica, Am. 

 J. Trop. Med. 7: 225, 1927. Complement Fixation in the Diagnosis 

 of Infections With Endameba Histolytica, Ibid. 8: 29, 1928, The 

 Technique and Kesults of a Complement Fixation Test for the 

 Diagnosis of Infection With Endameba Histolytica, Ibid, 9: 277, 

 1929. The Diagnostic Value of the Complement Fixation Test in 

 Amebic Infection, .1, A, M. A. 95: 10, 19.'?0. 



Downs, C. At.: Personal communication. 



Khrlich, Paul: Studies in Immunity, translated by Bolduan, Xew York, 

 1910, .lohn Wiley & Sons. 



Gay, F. P.: Typhoid Fever, New York, 1918, The Macmillan Co. 



Gengou, O. : Sur les sensibilistrices des .serums aetifs contre les substances 

 albuminoides, Ann. de I'lnst. Pasteur 16: 734, 1902. 



Kolmer, J. A., and Brown, C. P. : Studies in the Standardization of the 

 Wassermann Reaction. III. The Red Corpuscle Suspension for the 

 Wassermann Reaction. The Preservation of Red Blood Cells, Am. J. 

 Syph. 3: 169, 1919. 



Kolmer, J. A. : Infection, Immunity, and Biologic Therapy, ed. 3, Phila- 

 delphia, 1925, W. B. Saunders Co. 



Kolmer, J. A., and Boerner, F. : Approved Laboratory Technic, New York, 

 1941, D, Appleton-Century Co., p. 637. 



Kolmer, J, A.: Technics of Serodiagno.'^tic Te.sts for Syphilis, U. S. P. H. 

 Service, Supplement 11, p. 56, 1940. 



M<irgenroth, .1., and Sachs, H. : Cited bv Ehrlich in Studies in Immunity, 

 Bolduan, New York, 1910, ,Tohn W'iley & Sons, p, 250. 



