HYPERSENSITH^ENESS 519 



to MA-100 and OT in tuberculous and nontuberculous subjects. 

 In this work the skin reactions with OT and the MA-100 pi'otcins 

 of the timothy bacillus and tlie human, bovine and avian tubercle 

 bacillus were studied in 225 individuals by Funk, Huntoon and 

 White and in 3,454 by Mariette and Fenger. They employed the 

 following dosage in these investigations: 



Tuberculin OT "Dorset": Dose 0.05 c.c. = 0.00001 c.c. commonly 



designated at 0.01 mg. 

 MA-100: Human tubercle bacillus protein: 0.05 c.c. = 0.0005 mg, 

 MA-100: Bovine tubercle bacillus protein: 0.05 c.c. = 0.0001 mg, 

 MA-100: Avian tubercle bacillus protein: 0.05 c.c, = 0,001 mg. 

 MA-100: Timothy bacillus protein: 0,05 c,c, := 0,01 mg. 



If the reaction is negative at the end of forty-eight hours, repeat 



with double the dose. 



The statistics of Funk, Huntoon and White are fairly repre- 

 sentative of the general results and may be tabulated as follows: 



TEST MATERIAL 



Tuberculin OT "Dorset" 

 MA-100 Human tubercle protein 

 MA-100 Bovine tubercle protein 

 MA-100 Avian 

 MA-100 Timothy protein 



In a larger series studied by Mariette and Fenger, the ]\IA-100 

 human tubercle protein gave about 10 per cent more positives 

 among adults and 2 per cent among children classified as sana- 

 torium patients than tuberculin OT. In tlie general population 

 i\[ariette and Fenger report that tuberculin OT gave 27.2 per 

 cent positives among adults and 6.9 per cent positives among chil- 

 dren, whereas ]\IA-100 human tubercle protein yielded 39.9 and 8.5 

 per cent positives, respectively, in the same individuals. From 

 these studies they draw the following conclusions : 



1, That MA-100 human tubercle protein is probably more sensi- 

 tive than OT. 



2, The initial and repeat doses may be administered safely to 

 tuberculous patients. 



3, Larger doses are not necessary to detect the majority of tuber- 

 culous individuals, 



4, Large doses of MA-100 proteins give nonspecific results. 



5, There is a protein common to all members of the acid-fast 

 group which when administered in large doses gives a re- 

 action similar to a positive tuberculin (OT) reaction. 



G, They regard MA-100 tubercle protein as purer and better than 

 OT for determining tuberculin hypersensitiveness. 



