1 1 YPKKSEN.SITIVEX KSS 



527 



nous injection 

 Sensitizing antigen 



Testing antigen 



Tissue culture re- 

 sponse to antigen 



Ordinary proteins 



Bacteria and some of 

 their protein-contain- 

 ing products 



Proteins 



Carbohydrate fraction 

 of bacteria (appar- 

 ently the most effec- 

 tive) 



Transplants of bone 

 marrow and spleen 

 from sensitized ani- 

 mals not killed by 

 antigen 



Bacteria, best living but 

 also killed if in condi- 

 tion to produce granu- 

 lomatous tissue responses 

 in tuberculosis. Tuber- 

 culins MA-100 and TPT 



Bacterial proteins and pro- 

 tein fractions only 



Transplants of bone mar- 

 row and spleen from tu- 

 berculous animals killed 

 bv tuberculin 



crystalline egg albumen intraeutaneonsl.y, the resulting reaction is 

 specific and of the tuberculin tj'pc, i.e., it is delayed and prolonged. 

 When the sensitizing dose of egg albiunen is administered elsewhere 

 tiian directly into a tuberculous lesion, a skin test performed eight 

 to ten days later with the homologous antigen results in an im- 

 mediate and transitory or anaphylactic type of reaction. 



Certain reports in the literature of exceptions to these results 

 suggested to Dienes and Mallory that a time factor might be im- 

 portant. They, therefore, sensitized normal uninfected guinea 

 pigs to egg albumen and egg globulin and noted the type of re- 

 action when skin tests were made with the homologous antigen 

 at four-, six-, eight-, and ten-day intervals following the adminis- 

 tration of the sensitizing dose of antigen. They discovered that re- 

 actions obtained on the sixth day and earlier were of the tuberculin 

 type, while the reactions obtained later were of the anaphylactic 

 type. When skin tests were performed upon guinea pigs pas- 



