34 



NUCLEIC ACIDS AS CELL CONSTITUENTS 



TABLE 14 



APURINIC ACID FROM CALF THYMUS DNA 



DNA 



Apurinic 

 acid 



N 

 P. 



N/P 



Absorption maximum, m/i* 

 Absorption maximum, e (?) 

 Optical rotation** 

 Adenine, moles per mole P 

 Guanine, moles per mole P 

 Cytosine, moles per mole P 

 Thymine, moles per mole P 

 Thymine to cytosine 



* In M phosphate buffer, pH 7.1. 

 ** In 0.1 M phosphate buffer, pH 7.1. 



TABLE 15 



ACTION OF RIBONUCLEASE ON PNA 



Source Fraction 



Nucleotide composition 

 {as moles 1 100 M nucleotide in starting material) 



Guanylic Adenylic Cy tidy lie Uridylic j . j 

 acid acid acid acid 



Yeast 



Pig liver 



21 

 15 





 18 



19 



14 





 15 



23 



19 



14 

 8 



29 



26 



18 

 



26 100 



26 78 



19 33 



8 



16 100 



15 73 



10 28 



14 



