46 DEOXYPENTOSE NUCLEOPROTEINS AND PROSTHETIC GROUPS 



0.4 0.6 0.8 1.0 2.6 

 MNaCI 



Fig. 3. Fractional dissociation, in the presence of a CHCI3 phase, of 

 artificial complexes between the deoxyribonucleic acids of calf thymus 

 (curves A and B) and pig liver (curve C) with globin (curve A) or histone 

 (curves B and C). Co-ordinates as in Fig. 2. (Taken from Crampton 



et fl/.6.) 



such as chloroform-pentanol, a large number of distinct nucleic 

 acid fractions can be separated, as will be discussed below. The 

 fractional dissociation of calf thymus nucleohistone and of the 

 nucleoprotein of wheat germ, based on previous work^ and on 

 unpublished experiments with Miss Rakoma Lipshitz, is shown 

 in Fig. 2. These are curves taken from single experiments; but 

 the typical shape can be reproduced easily. 



e. Behavior of artifacts 



The behavior of artificially prepared complexes of nucleic acids 

 with positively charged substances has also been studied. Such 

 nucleate compounds have been prepared not only with histone 

 and globin^, but also with synthetic polyelectrolytes, e.g., polyly- 

 sine or polyvinylamine^^. The fractional dissociation of deoxy- 

 ribonucleate complexes with globin or histone is illustrated in 



