OXIDASES, PEROXIDASES, AND CATALASE 



101 



Figure 3). If our concept of the mechanism of the Pasteur reaction 

 is correct, it should be possible to replace the dyestuffs in Lipmann's 

 experiments by an autoxidizable hemochromogen of suitable poten- 

 tial. Pyridine, histidine, and picoline hemochromogen have been 

 tried without positive results. The experiments with the very posi- 

 tive systems nicotine and nicotinic acid amide hemochromogen, on 



PASTEUR 

 ENZYME 



FERMENTATION 

 ENZYME SYSTEM 



SUBSTRATE 



Nicotine Fern- 



Hemochromogen 



Eo=>*0.200v.(pH6.0) 



FERMENTING 

 YEAST MACERATION 

 EXTRACT 



["glucose 



<^fructose 



[sucrose 



Figure 3. — Attempts at reconstruction of Pasteur reaction in cell-free systems 



the other hand, have yielded some encouraging results which, un- 

 fortunately, did not prove to be reproducible at will. One has the 

 impression that the underlying idea is correct but that some of the 

 factors involved in the complex reaction have thus far evaded 

 adequate control. 



The problem of the Pasteur reaction, as the writer sees it, is now 

 shifting from the nature of the catalyst to the nature of its substrate. 



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4. Barron, E. S. C, Physiol. Rev., 19, 184 (1939). 



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