NICOTINAMIDE NUCLEOTIDE ENZYMES 



117 



Depending on the experimental conditions, the product from 

 alkaline hydrolysis was nicotinamide (28) or adenosine diphosphoric 

 acid (29), the structure of which is well established by the work of 

 Lohmann (30), Embden (31), and Levene (32). Alkaline hydrolysis 

 in the cold gives, besides nicotinamide, a product of adenosine 

 diphosphate plus pentose (formula 7). This degradation product 

 of cozymase is inactive as coenzyme (33). 



N = C-NH 



I I 



HC C— N\ 



II II ^CH 

 N — C — N 



FORMULA 7 



CONH. 



,^ 



OH OH H OH OH 



II 11/ 



-C— C — C — C — c-o-p-o-p=o 



I I I I I II \ 



H H H H H O OH 



OH OH 



I I 

 Adeni ne — pentose — P-0-P-peniose -f- 



II II 

 O O -N 



The enzymatic hydrolysis gave the two nucleosides: 



FORMULA 8 



^ 



■CONH. 



.^ 



I 



I 



N 



H 



I 



X- 



C- 



I 

 N 



■CONH- 



■N 

 II 

 ■C-NH. 



HC 



I 

 HCOH 



I 

 HCOH 



I 

 HC 



^C^ 



O 



HC- 



I 

 HCOH 



I 

 HCOH 



O 



HC 



I 



CH^OH 



CH^OH 



