NICOTINAMIDE NUCLEOTIDE ENZYMES 119 



II might contain adenosine triphosphate as a part of its molecule 

 (formula 10, 1). 



FORMULA 10 



O OH OH 



© III 



I Nicofin amide -pentose — P— O— P— O— P — pentose- adenine 



11 II II 







®0 OH 



® I I 



2. Nicotinamide- pentose — P — O — P — pentose- adenine 



o o 'p^hI 



This hypothesis, however, was not substantiated by experimental 

 results. Experiments carried out with rather limited amounts of pure 

 coenzyme II showed that it contains no readily hydrolyzable phos- 

 phate, and yields no alkaline degradation product active as a co- 

 phosphorylase (34). Therefore it seems probable that the third phos- 

 phoric acid group is linked to the adenylic acid part of the molecule, 

 perhaps in much the same way as in yeast adenylic acid (formula 

 10, 2). This phosphoric acid group should block the coenzyme prop- 

 erties of the adenylic acid part when liberated by alkaline hydrolysis. 

 Further experimental work is needed to decide this question. 



The small amounts of adenosine-5' -phosphoric acid and its homo- 

 logues which occur as contaminants of impure codehydrogenase 

 preparations and which are formed from alkaline hydrolysis of 

 cozymase but not from codehydrogenase II can be traced by a test 



FORMULA 11 



GOGH GOOH GOOH 



1 , I /OH _x I /OH 



GHOH > GH-0-P=0 7 G-0-P=0 



I /OH < I ^oH <— N. OH 



GH 0-P=0 GHOH GH^ 



OH 



GOOH GOOH 



2 C-P^o'~' 4- ADENYLIC \ p C-OH + ADENOSINE- 



II Vu ACID II TRIPHOSPHATE 



GH^^ GH^ 



ADENOSINE- TRIPHOSPHATE — > ADENYLIC ACID 

 + 2 PHOSPHORIC ACID 



