HAEMOSPORIDIA 441 



H. lophortyx O'Roke (Fig. 200, n-s). In California Valley quail, 

 Lophortyx spp. ; gametocytes in erythrocytes, also occasionally in 

 leucocytes; young gametocytes, spherical to elongate, about Ifi 

 long; more developed forms, cylindrical, about S/jl by 2/a, with 2- 

 10 pigment granules; mature gametocytes, halter-shaped, en- 

 circling nucleus of host erythrocyte, 18yu by 1.5-2.5ai; numerous 

 pigment granules; 4-8 microgametes, about 13.5/x long, from each 

 microgametocyte; on slide in one instance, gamete-formation, 

 fertilization and ookinete formation, completed in 52 minutes at 

 room temperature; in nature sexual reproduction takes place in 

 the fly, Lynchia hirsuta, which process seems to be similar to that 

 of Plasmodium in mosquitoes (p. 435); sporozoites enter salivary 

 glands and fill central tubules; schizonts present in lungs, liver 

 and spleen of quail after infected flies sucked blood from the bird; 

 merozoites found in endothelial cells of capillaries of lungs, in 

 epithelial cells of liver and rarely in peripheral blood cells ; how 

 merozoites enter blood cells is unknown; schizonts seldom seen in 

 circulating blood ; infected birds show pigment deposits in spleen 

 and lungs (O'Roke). 



Genus Leucocytozoon Danilewsky. Schizogony in endothelial 

 cells and cells of viscera of vertebrates; sexual reproduction in 

 blood-sucking insects; gametocytes occur in spindle cells or retic- 

 ulocytes. 



L. anatis Wickware (Fig. 201). In wild and domestic ducks; 

 sexual cycle in the black fly, Simulium venustum. O'Roke (1934) 

 studied the life-cycle of this sporozoan: gametocytes develop into 

 mature gametes in 1-2 minutes after blood is obtained from an 

 infected duck; macrogametes about S^u in diameter; 4-8 micro- 

 gametes, 15.7-24.1^ long, from a single microgametocyte; zygotes 

 are found in stomach contents of fly in 10-20 minutes after suck- 

 ing in infected blood of bird; motile ookinetes abundant after 5 

 hours, measure 33.3/i by 3-4. 6ju; 22 hours after sucking duck 

 blood, oocysts found on outer wall of stomach; sporozoites ma- 

 ture probably in 24-48 hours; 5 days after a duck has been bitten 

 by infected black flies, schizogonic stages are noticed in endo- 

 thelial cells of capillaries of lungs, liver, spleen; on about 7th day 

 gametocytes appear in blood; liver and spleen become hyper- 

 trophied; the infection among ducklings is said to be highly fatal 

 and appears often suddenly. 



L. simondi Mathis et Leger. Macrogametocytes oval, 14-15/1 



