Contents 



Contributors to Volume II v 



Contents of Volume I xi 



16. The Nucleic Acid Content of Tissues and Cells by I. Leslie 1 



I. Introduction 1 



II. Methods 2 



III. Normal Adult Tissues 7 



IV. Embryonic Development and Postnatal Growth 15 



V. Regenerating Tissues and Tissue Explants 19 



VI. Dietary Factors Influencing Nucleic Acid Content 24 



VII. Neoplastic Tissues and Chemical Carcinogenesis 27 



VIII. Hormonal Influences 32 



IX. Various Pathological Conditions 39 



X. Plant Tissues 40 



XI. Bacteria and Viruses 41 



XII. Conclusion 43 



XIII. Addendum 44 



17. Cytochemical Techniques for Nucleic Acids by Hewson Swift 51 



I. Introduction 51 



II. Basic Dyes 52 



III. The Feulgen Reaction 65 



IV. Other Cytochemical Reactions 78 



V. Extraction Techniques 78 



VI. Photometric Methods for Cell Studies 82 



VII. Ultraviolet Absorption 84 



VIII. Conclusions 89 



IX. Methods 90 



18. The Isolation and Composition of Cell Nuclei and Nucleoli by Alexander 



L. Dounce 93 



I. Methods of Isolating Cell Nuclei 94 



II. Chemical Composition of the Cell Nucleus 120 



III. The Nucleolus and Nucleolar PNA 143 



19. The Deoxyribonucleic Acid Content of the Nucleus by R. Vendrely 155 



I. Introduction 155 



II. The DNA Content of the Nucleus 157 



III. Can the DNA Content of the Nucleus Be Considered as a "Constant?" 168 



IV. Conclusion 176 



20. Nucleic Acids in Chromosomes and Mitotic Division by B. Thorell 181 



I. Introduction 181 



II. The Chromosomal Nucleic Acids 182 



III. Changes in the Chromosomal Nucleic Acids During the Cell Cycle. . 192 



IV. Concluding Remarks 198 



vii 



