98 



ALEXANDER L. BOUNCE 



Receptacle 



Detail of 

 Perforated Plates 



Fig. 1. Apparatus for removing fiber from tissue, 0.6 times actual size; construc- 

 tion of stainless steel. For small quantities of tissue, a smaller top piece and plunger B 

 can be constructed to fit the same perforated plates and collar used for the top piece 

 shown in A. In this case not all the holes in the perforated plates will be used. The 

 larger perforated plate has 37 holes of j'g inch diameter; the smaller upper plate has 

 3 holes of 3^2 inch diameter over one 3-^-inch hole in the larger plate, as shown in A. 



lowered to 6 or to 4 or even lower with citric acid;^"^^ or calcium chloride 

 can be added. '^ Otherwise, when the Blendor is run at a high enough speed 

 to break up the cells, the nuclei will also be disintegrated. In the second 

 place, if the Blendor is run at a high enough speed to break up 95 % or more 

 of the cells, the mitochondria will also be broken, and, if the Blendor is 

 run at a lower speed, the residual whole cells concentrate with the nuclei 



8 A. L. Bounce, J. Biol. Chem. 151, 221 (1943). 



9 A. L. Bounce, /. Biol. Chem. 151, 235 (1943). 

 '«A. Marshak, J. Gen. Physiol. 25, 275 (1941). 



11 F. L. Haven and S. R. Levy, Cancer Research 2, 797 (1942). 



12 A. E. Mirsky and A. W. Pollister, J. Gen. Physiol. 30, 117 (1946). 

 " C. Vendrely, Bull. biol. France et Belg. 86, 1 (1952). 



" R. M. Schneider and M. L. Peterman, Cancer Research 10, 751 (1950). 



