392 GEORGE BOSWORTH BROWN AND PAUL M. ROLL 



a single perchloric acid extract of rat tissues has been demonstrated^^^ (cf. 

 footnote 171). The separation of an adenosine tetraphosphate (with three 

 labile phosphates) from commercial ATP is reported. ^^° 



Possible anabolic routes for nucleosides and adenine are suggested by 

 newly recognized enzymatic activities. A preparation from malt diastase 

 will accomplish phosphorylation of ribo- or deoxyribonucleosides by trans- 

 fer of phosphate from phenylphosphate.^^' The incorporation of adenine 

 directly into a nucleotide (AMP) through the action of ribose-l,5-di phos- 

 phate and a pigeon liver nucleotide phosphorylase is described. ^^^ 



Demonstration of some incorporation of glycine and adenine into the 

 DNA of mature sea urchin sperm, in vitro, gives direct evidence of the for- 

 mation of DNA in the absence of mitosis. ^^* In brain slices an incorporation 

 of inorganic P^^ into the PNA nucleotides, but little (and that of doubtful 

 validity) into the DNA, was observed,^^* which is in harmony with the re- 

 sults with formate in vivo^^ rather than with the earlier ones with phos- 

 phate.i" 



Studies of the incorporation of the 2'- and 3'-phosphates of cytidine have 

 shown that they are equally readily incorporated into the nucleic acids of 

 regenerating rat liver.^^^ 



Further reports have appeared on the incorporation of purines into T. 

 gelW^^ (cf. footnote 83), on the incorporation in vivo of 2,6-diaminopurine 

 into a phosphate derivative of its nucleoside^^^ (cf. footnote 170), and on 

 the incorporation in vivo of adenine into soluble nucleotides.^'* (cf. footnote 

 215). 



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66 (1954). 

 "0 D. H. Marrian, Biochim. et Biophys. Acta 12, 492 (1953). 

 "1 G. Brawerman and E. Chargaff, J. Am. Chem. Soc. 75, 2020 (1953). 

 "2 M. Saffran and E. Scarano, Nature 172, 949 (1953). 

 233 H. M. Malkin, Biochim. et Biophys. Acta 12, 585 (1953). 



"' H. A. Deluca, R. J. Rossiter, and K. P. Strickland, Biochem. J. 55, 193 (1953). 

 «s p. Reichard, Acta Chem. Scand. 7, 862 (1953). 

 "6 M. Flavin and M. Engelman, ./. Biol. Chem. 200, 59 (1953). 

 237 G. P. Wheeler and H. E. Skipper, J. Biol. Chem. 205, 749 (1953). 

 "8 E. L. Bennett, Biochim. et Biophys. Acta 11, 487 (1953). 



