CONTENTS 



Foreword vii 



Preface ix 



Conventions xiii 



Table of Symbols xiii 



CHAPTER 1 



Perspectives of Metabolic Inhibition 



CHAPTER 2 



The Kinetics of Enzyme Reactions 



The Dependence of Enzyme Rate on the Substrate Concentration 



Vahdity of the MichaeHs-Menten Theory 



Nature of the Intermediate Complexes with the Enzyme 

 Factors Determining the Rate of Enzyme Reactions . 

 Kinetics of Some Complex Enzyme Reaction Types 



13 \i 



14 

 17 

 27 

 31 

 32 



CHAPTER 3 



The Kinetics of Enzynne Inhibition 



Reversible and Irreversible Types of Inhibition 

 Competitive and Noncompetitive Inhibitors 

 Generalized Kinetics of Reversible Inhibition . 

 Inhibition Kinetics in Mutual Depletion Systems 

 Pseudo-Irreversible Inhibition .... 

 Inhibition by Reaction with Coenzyme or Activator Sites 

 Inhibition by Reaction with Substrate, Coenzyme, or Activator . 

 The Enzyme Combines with More than One Molecule of Inhibitor 

 The Inhibitor Is Also a Substrate for the Enzyme .... 

 Some Factors Occasionally Important in Inhibition 

 Expression and Interpretation of Inhibition 



49 



52 

 54 

 55 

 66 



78 

 82 

 85 

 90 

 96 

 98 

 104 



CHAPTER 4 



Substrate Inhibition and Product Inhibition 



Substrate Inhibition 

 Product Inhibition . 



HI 



111 

 140 



XVll 



r^. 



19 G 



