CONTENTS 



XIX 



Transition between Steady States 



The Future of the Studv of Multienzvme Systems 



383 

 391 



CHAPTER 8 



Distribution and Fate of Inhibitors in Living Organisms 



Distribution of Inhibitors in Animals 



Distribution of Inhibitors in Phmts 



Distribution of Inhibitors within Cells 



Fate of Inhibitors in the Animal 



Repeated Administration of an Inhibitor 



Distribution and Effect of Inhibitors 



Chemical Composition of the Media 



Distribution and State of Enzymes in Cells 



393 



394 

 405 

 410 

 413 

 421 

 425 

 428 

 432 



CHAPTER 9 



Inhibition in Cells and Tissues 



Types of Inhibition Unique to Organized Systems . 



The Problem of Endogenous Metabolism 



Inhibitor-Stable Metabolism 



Stimulation of Metabolism by Inhibitors 



Intracellular Equilibria and Secondary Effects of Inhibitors . 

 The Concept of Pacemakers in Metabolism .... 

 Relations between Enzyme Inhibition and Changes in Cell Funct 

 Quantitative Expression of Intracellular Enzyme Inhibition . 

 Inhibition in Tissue Slices and Isolated Tissue Preparations . 

 Differential Effects of Inhibitors in Whole Animals . 

 Enzyme Variability 



427 



436 

 441 

 447 

 451 

 456 

 458 

 462 

 474 

 479 

 481 

 485 



CHAPTER 10 



Effects of More than One Inhibitor 



Two Inhibitors Acting on a Single Enzyme 



Two Inhibitors Acting on a Monolinear Chain . 



Two Inhibitors Acting on Various Multienzyme Systems 



Two Inhibitors Acting on Cellular and Tissue Metabolism 



Antagonism, Summation, and Synergism .... 



Specificity of Inhibition with Two Inhibitors . 



Inhibition of Bioluminescence with Sulfanilamide and Urethane 



487 



488 

 498 

 500 

 502 

 507 

 510 

 510 



CHAPTER 11 



Localization of the Site of Inhibition 



Methods of Localization 



General Deyelopment of a Localization Program 



513 



513 

 527 



