16 



2. THE KINETICS OF ENZYME REACTIONS 



graphically by plotting Ijv against 1/(S) since the reciprocal form of the 

 Michaelis-Menten equation: 



1 K 



(2-8) 



80 



60- 



40- 



♦ 20l- 



0.01 



100 ntM 



Fig. 2-2. Plots of Eq. 2-7 for different values of the Michaelis constant. F„ = 100. 



requires that such a plot will result in a straight line of slope KIVj„ and will 

 intersect the Ijv axis at 1/F,„. The value of K may be found directly by 

 extending the plot to the intersection with tlie 1/(S) axis, this being IjK 

 (Dixon, 1953 a) (Fig. 2-3). 



Fig. 2-3. Reciprocal plot of the Michaelis-Menten 

 equation for the purpose of determining A',„ and V„. 



