CxEXERALIZED KINETICS OF REVERSIBLE INHIBITION 



63 



ing must be reduced markedly, before the influence of changes in /? is negli- 

 gible. In Figs. 3-7 and 3-8 it is evident that inhibition ceases and activation 

 occurs where the lines cross the /5-axis; for example, when or = 30 (i.e., 



ps- 



FiG. 3-5. Variation of the fractional inhibition with .substrate concentration for 

 different types of inhibition. Constants and curve designations as in Fig. 3-2. 



0.1 



10 



100 



Fig. 3-6. Variation of the fractional inhibition with the interaction constant a 

 for different values of ^. K, = 1 mJ/, A', = 0.1 m3/, (S) = 3 mM. and (I) = 0.3 mM. 



the Kg is increased thirty-fold by the inhibitor), the rate of substrate com- 

 plex breakdown must be accelerated over eight-fold for stimulation of the 

 reaction to occur. These curves clearlv show that the resulting inhibition 



