MUTUAL DEPLETION SYSTEMS 



73 



where a = fractional activity as defined by Goldstein. It is particularly 

 important to note that this fractional activity is not equal to \—i as it 

 is usually defined. As used by Goldstein, a=(ES)/(E,) = V(/F„; and hence re- 



FiG. 3-12. Illustration of the method of 

 Easson and Stedman (1936) for the deter- 

 mination of enzyme concentration from 

 inhibition data. The plot is of Eq. 3-42. 



presents the activity relative to the maximal rate, not relative to the actual 

 rate in the absence of inhibitor, in which case the fractional activity would 

 be equal to vjv. In other words, the fractional activity of Goldstein does 



Fig. 3-13. Illustration of the method of 



Myers (1952 a) for the determination of 



enzyme concentration from inhibition data. 



The plot is of Eq. 3-44. 



not express the reduction of rate due to inhibitor alone. It is easy to show 

 that: 



(1 - O(S') 

 (S') + 1 



(3-47) 



