GENERALIZED EQUATIONS FOR DETERMINATION OF CONSTANTS 155 



components of the system merely modify the rate), complexes other than 

 ES may be active and the rate expressions will be similar to that above 

 but will involve additional terms for each active complex. If we take Xj 

 to be an inhibitor, the six equations used in plotting may be written as: 



K, 



rjs) 



_(S)_ 

 F,„ 



_(I)_ (X.J 



(I) 



+ 



(5-13) 



(xj - 



vKn . 



(5-14) 



Type C: 



Vi 



VzKs 



(S) 



(I) , (XJ 



(I) (XJ 



^-t4 (5-15) 



+ ... + ^ 



+ 



(I) 



"F,„A^ 



__(X^ 



(XJ I 



'A„ J 



(5-16) 



Type E: 



aK, 



(I) 



(S) 

 A, 



^ 1 



(X,) (XJ , (S) 



A% "^■■■^ A„ A, 



1 + 



(X,) 

 iSA, 



(XJ 

 (5-17) 



Type F: 

 (I)(l -i] 



uK, 



a + 



(S) 



(X,) 

 A. 



(XJ , (8) 

 A„ A. 



1 + 



(X,) 



•...+ 



(X„ 



iA„ 



(5-18) 



When more than one substrate, inhibitor, or other component molecule 

 reacts with the enzyme, the equations must be correspondingly modified 



