MUTUAL DEPLETION SYSTEMS 



187 



E + I -^ EI + X: 



1 



K,[l +(S)/AM 



(I.)(l -i) 



+ i 



(5-39) 



These equations are modified from those of Kriipka and Laidler merely by- 

 substituting i for 1 — vjv. Equation 5-38 is seen to be the same as 3-50 

 for zone Afi^ inhibition. In the first case, (/,) — i{Ei) is plotted against 

 il{\ — i), and in the second case, i is plotted against the expression in the 

 square brackets. In both cases, Kj may be readily evaluated. They applied 

 these kinetics to the inhibition of a-chymotrypsin by diisopropylfluoro- 

 phosphate (DFP), and found that at low inhibitor concentrations the first 



Fig. 5-24. Mutual depletion system in zone C,-. 



