356 



7. INHIBITION IN MULTIENZYME SYSTEMS 



oxidation-reduction reactions, X represents hydrogen atoms or electrons, 

 and for transphosphorylations, X represents phosphate. The kinetics of 

 these systems and their response to inhibition are very interesting and 

 of great importance in understanding cellular metabolic pathways and 

 their modification. 



008 



— 06 



— 04 



- 002 



(A) 

 (B) 

 (C) 



Fig. 7-22. Noncompetitive inhibition of Ej in the cycHc system shown 

 in Fig. 7-16. (M)^ = 0.1 milf. 



In the steady state v^ — v.^ where these rates are given by: 



^ F,(AX)(B) 



"^ [(AX) + A'J [(B) + K,] 



^ F,(BX)(C) 



"^ [(BX) + A',,] [(C) + k;\ 



where B is a dissociable linking cofactor. Solving for (BX): 



(BX) 



1 + 



(B) 



1 4- 



TaxT 



(C) 

 (C) + K, 



F: 



(7-40) 

 (7-41) 



(7-42) 



